Technical Capabilities

Know How

Name: Generation of induced pluripotent stem cells (iPSCs).
Main applications: Experimental models of gene and cell therapy
Equipment required: Level 2 containment cell culture laboratory, biosafety hoods, incubators, microscopes, ultra centrifuge, ultrafreezer
Description: We generate induced pluripotent stem cells by the transduction of mouse fibroblasts with Klf4, Oct4, Sox2 and c-Myc.

Name: Generation of induced pluripotent stem cells (iPSCs)
Main applications: Experimental models of gene and cell therapy
Equipment required: Level 2 containment cell culture laboratory, biosafety hoods, incubators, microscopes, ultra centrifuge, ultrafreezer
Description: We generate induced pluripotent stem cells by the transduction of mouse fibroblasts with Klf4, Oct4, Sox2 and c-Myc.

Name: Cytometry and cell sorting laboratory, LACISEP
Main applications: Analysis and separation of cell populations (hematopoietic stem cells, etc.)
Equipment required: Flow cytometer, cell separator, laminar flow, fluorescence microscope, centrifuge and refrigerator
Description: Development of protocols for flow cytometric analysis and cell sorting by FACS.

Name: Culture of human and mouse hematopoietic progenitors.
Main applications: Quantification of progenitors in hematological samples (bone marrow, spleen).
Equipment required: Incubators, laminar flow, fluorescence inverted microscope, centrifuges and refrigerators
Description: To study hematopoiesis is crucial the culture of hematopoietic progenitors in semisolid media and subsequent identification.

Name: Analysis of mouse repopulating cells
Main applications: Quantification and analysis of hematopoietic stem cells
Equipment required: Animal house, built insulators, irradiator, incubators, laminar flow, fluorescence inverted microscope, centrifuges and refrigerators
Description: To study hematopoiesis is essential the quantification and analysis of hematopoietic stem cells capable of long-term reconstitution. Mouse cells and transplantation of human hematopoietic cells in immunodeficient mice are used.

Name: Laboratory for sample handling in clinical use-GMP laboratory
Main applications: Clinical protocols
Equipment required: Clean room, incubators, biosafety cabinets, centrifuge, CliniMacs, etc..
Description: We have the technology and equipment for the genetic manipulation of cells from patients for clinical use.

Models

Description: We have several animal models for the study of Fanconi anemia.
Biological functions affected: Biochemistry and hematology, immune system.

Description: We have an animal model for erythrocyte pyruvate kinase deficiency.
Biological functions affected: Biochemistry and hematology.

Description: We have several animal models for the study of immune deficiencies, specifically SCID, NOD/SCID/gamma-Cnull, LAD deficient animals.
Biological functions affected: Immune system.

Description: We have lymphoblastoid cell lines and fibroblasts derived from patients with Fanconi anemia.

Description: We have several animal models for the study of inflammatory bowel diseases (acute and chronic).
Biological functions affected: Immune system.