Manzanares, P.; Ballesteros, I.; Negro, M.J.; González, A.; Oliva, J.M.; Ballesteros, M. Processing of extracted olive oil pomace residue by hydrothermal or dilute acid pretreatment and enzymatic hydrolysis in a biorefinery context. Renewable Energy, 2020, 145:1235-1245. Ver Publicación.

Moreno, A.D.; Ibarra, D.; Eugenio, M.E.; Tomás‐Pejó, E. Laccases as versatile enzymes: From industrial uses to novel applications. Journal of Chemical Technology and Biotechnology, 2020, 95(3):481-494. Ver Publicación.

Cubas-Cano, E.; López-Gómez, J.P.; González-Fernández, C.; Ballesteros, I.; Tomás-Pejó, E. Towards sequential bioethanol and l-lactic acid co-generation: Improving xylose conversion to L-lactic acid in presence of lignocellulosic ethanol with an evolved Bacillus coagulans. Renewable Energy, 2020, 153:759-765. Ver Publicación.

Padilla-Rascón, C.; Ruiz, E.; Romero, I.; Castro, E; Oliva, J.M.; Ballesteros, I.; Manzanares, P. Valorisation of olive stone by-product for sugar production using a sequential acid/steam explosion pretreatment. Industrial Crops and Products, 2020, 148:112279. Ver Publicación.

Geijer, C.; Faria-Oliveira, F.; Moreno, A.D.; Stenberg, S.; Mazurkewich, S.; Olsson, L. Genomic and transcriptomic analysis of Candida intermedia reveals the genetic determinants for its xylose-converting capacity. Biotechnology for Biofuels, 2020, 13:48. Ver Publicación.

Cubas-Cano, E.; González-Fernández, C.; Ballesteros, M.; Tomás-Pejó, E. Lactobacillus pentosus CECT 4023 T coutilizes glucose and xylose to produce lactic acid from wheat straw hydrolysate: Anaerobiosis as a key factor. Biotechnology Progress, 2019, 35(1):e2739. Ver Publicación.

Fernández, J.L.; Sáez, F.; Castro, E.; Manzanares, P.; Ballesteros, M.; Negro, M.J. Determination of the lignocellulosic components of olive tree pruning biomass by near infrared spectroscopy. Energies, 2019, 12:2497. Ver Publicación.

González-Fernández, C.; Méndez, L.; Tomás-Pejó, E., Ballesteros, M. Biogas and volatile fatty acids production: Temperature as a determining factor in the anaerobic digestion of Spirulina platensis. Waste and Biomass Valorization, 2019, 10(9):2507-2515. Ver Publicación.

Moreno, A.D.; González-Fernández, C.; Ballesteros, M.; Tomás-Pejó, E. Insoluble solids at high concentrations repress yeast’s response against stress and increase intracellular ROS levels. Scientific Reports, 2019, 9:12236. Ver Publicación.

Álvarez, C.; Sáez, F.; González, A.; Ballesteros, I.; Oliva, J.M.; Negro, M.J. Production of xylooligosaccharides and cellulosic ethanol from steam-exploded barley straw. Holzforschung, 2019, 73(1):35–44. Ver publicación.

Moreno, A.D.; Carbone, A; Pavone, R; Olsson, L; Geijer, C. Evolutionary engineered Candida intermedia exhibits improved xylose utilization and robustness to lignocellulose-derived inhibitors and ethanol. Applied Microbiology and Biotechnology, 2019, 103(3):1405-1416. Ver publicación.

Solarte-Toro, J.C.; Romero-García, J.M.; Susmozas, A.; Ruiz, E.; Castro, E.; Cardona-Alzate; C.A. Techno-economic feasibility of bioethanol production via biorefinery of olive tree prunings (OTP): optimization of the pretreatment stage. Holzforschung, 2019, 73(1):3–13. Ver publicación.

Susmozas, A.; Moreno, A.D.; Romero-García, J.M.; Manzanares, P.; Ballesteros, M. Designing an olive tree pruning biorefinery for the production of bioethanol, xylitol and antioxidants: a techno-economic assessment. Holzforschung, 2019, 73(1):15–23. Ver publicación.

Barreiro-Vescovo, S.; de Godos, I.; Tomás-Pejó, E., Ballesteros, M.; González-Fernández, C. Effect of microalgae storage conditions on methane yields. Environmental Science and Pollution Research, 2018, 25:14263–14270. Ver publicación

Cubas-Cano, E.; González-Fernández, C.; Ballesteros, M.; Tomás-Pejó, E. Biotechnological advances in lactic acid production by lactic acid bacteria: lignocellulose as novel substrate. Biofuels, Bioproducts and Biorefining, 2018, 12(2):290-303. Ver publicación

Duque, A.; Manzanares, P.; González, A.; Ballesteros, M. Study of the application of alkaline extrusion to the pretreatment of eucalyptus biomass as first step in a bioethanol production process. Energies, 2018, 11:2961. Ver publicación

González-Fernández, C.; Barreiro-Vescovo, S.; de Godos, I.; Fernández, M.; Zouhayr, A.; Ballesteros, M. Biochemical methane potential of microalgae biomass using different microbial inocula. Biotechnology for Biofuels, 2018, 11:184. Ver publicación

Guerrero, A.B.; Ballesteros, I.; Ballesteros, M. The potential of agricultural banana waste for bioethanol production. Fuel, 2018, 213:176–185. Ver publicación

Lara-Serrano, M.; Sáez Angulo, F.; Negro, M.J.; Morales-delaRosa, S.; Campos-Martin, J.M.; Fierro, J.L.G. Second-generation bioethanol production combining simultaneous fermentation and saccharification of IL-pretreated barley straw. ACS Sustainable Chemistry & Engineering, 2018, 6(5):7086-7095. Ver publicación

Magdalena, J.A.; Ballesteros, M.; González-Fernández, C. Efficient anaerobic digestion of microalgae biomass: Proteins as a key macromolecule. Molecules, 2018, 23:1098. Ver publicación

Manfredi, A.P.; Ballesteros, I.; Sáez, F.; Perottia, N.I.; Martíneza, M.A.; Negro, M.J. Integral process assessment of sugarcane agricultural crop residues conversion to ethanol. Bioresource Technology, 2018, 260:241–247. Ver publicación

Montipó, S.; Ballesteros, I.; Fontana, R.C.; Liu, S.; Martins, A.F.; Ballesteros, M.; Camassola, M. Integrated production of second generation ethanol and lactic acid from steam-exploded elephant grass. Bioresource Technology, 2018, 249:1017–1024. Ver publicación

Tian, H.; Fotidis, I.A.; Mancini, E.; Treu, L.; Mahdy, A.; Ballesteros, M.; González-Fernández, C.; Angelidaki, I. Acclimation to extremely high ammonia levels in continuous biomethanation process and the associated microbial community dynamics. Bioresource Technology, 2018, 247:616–623. Ver publicación

Álvarez, C.; González, A.; Negro, M.J.; Ballesteros, I.; Oliva, J.M.; Sáez, F. Optimized use of hemicellulose within a biorefinery for processing high value-added xylooligosaccharides. Industrial Crops and Products, 2017, 99:41–48. Ver publicación

Duque, A.; Manzanares, P.; Ballesteros, M. Extrusion as a pretreatment for lignocellulosic biomass: Fundamentals and applications. Renewable Energy, 2017, 114:1427-1441. Ver publicación

Fillat, U.; Ibarra, D.; Eugenio, M.E.; Moreno, A.D.; Tomás-Pejó, E.; Martín-Sampedro, R. Laccases as a potential tool for the efficient conversion of lignocellulosic biomass: A review. Fermentation, 2017, 3(2):17. Ver publicación

Guerrero, A.B.; Ballesteros, I.; Ballesteros, M. Optimal conditions of acid-catalysed steam explosion pretreatment of banana lignocellulosic biomass for fermentable sugar production. Journal of Chemical Technology and Biotechnology, 2017, 92:2351-2359. Ver publicación

Mahdy, A.; Fotidis, I.A.; Mancini, E.; Ballesteros, M.; González-Fernández, C.; Angelidaki, I. Ammonia tolerant inocula provide a good base for anaerobic digestion of microalgae in third generation biogas process. Bioresource Technology, 2017, 225:272–278. Ver publicación

Manzanares, P.; Ruiz, E.; Ballesteros, M.; Negro, M.J.; Gallego, J.; López-Linares, J.C.; Castro, E. Residual biomass potential in olive tree cultivation and olive oil industry in Spain: valorization proposal in a biorefinery context. Spanish Journal of Agricultural Research, 2017, 15(3):e0206. Ver publicación

Moreno, A.D.; Tellgren-Roth, C.; Soler, L.; Dainat, J.; Olsson, L.; Geijer, C. Complete genome sequences of the xylose-fermenting Candida intermedia strains CBS 141442 and PYCC 4715. Genome Announcements, 2017, 5(14):e00138-17. Ver publicación

Oliva, J.M; Negro, M.J.; Manzanares, P.; Ballesteros, I.; Chamorro, M.A.; Sáez, F.; Ballesteros, M.; Moreno, A.D. A sequential steam explosion and reactive extrusion pretreatment for lignocellulosic biomass conversion within a fermentation-based biorefinery perspective. Fermentation, 2017, 3(2):15. Ver publicación

Ruiz, E.; Romero-García, J.M; Romero, I.; Manzanares, P.; Negro, M.J.; Castro, E. Olive-derived biomass as a source of energy and chemicals. Biofuels, Bioproducts and Biorefining, 2017, 11(6):1077-1094. Ver publicación

Sanz, J.L.; Rojas, P.; Morato, A.; Méndez, L.; Ballesteros, M.; González-Fernández, C. Microbial communities of biomethanization digesters fed with raw and heat pre-treated microalgae biomasses. Chemosphere, 2017, 168:1013–1021. Ver publicación

Tomás-Pejó, E.; Fermoso, J.; Herrador, E.; Hernando, H.; Jiménez-Sánchez, S.; Ballesteros, M.; González-Fernández, C.; Serrano, D.P. Valorization of steam-exploded wheat straw through a biorefinery approach: Bioethanol and bio-oil co-production. Fuel, 2017, 199:403–412. Ver publicación

Alvira, P.; Negro, M.J.; Ballesteros, I.; González, A.; Ballesteros, M. Steam explosion for wheat straw pretreatment for sugars production. Bioethanol, 2016, 2:66–75. Ver publicación

Cardoso Coimbra, M.; Duque, A.; Sáez, F.; Manzanares, P.; García-Cruz, C.H.; Ballesteros, M. Sugar production from wheat straw biomass by alkaline extrusion and enzymatic hydrolysis. Renewable Energy, 2016, 86:1060–1068. Ver publicación

Da Silva, A.S.A.; Fernandes de Souza, M.; Ballesteros, I.; Manzanares, P.; Ballesteros, M.; Bon, E.P.S. High-solids content enzymatic hydrolysis of hydrothermally pretreated sugarcane bagasse using a laboratory-made enzyme blend and commercial preparations. Process Biochemistry, 2016, 51(6):1561–1567. Ver publicación

González-Fernández, C.; Mahdy, A.; Ballesteros, I.; Ballesteros, M. Impact of temperature and photoperiod on anaerobic biodegradability of microalgae grown in urban wastewater. International Biodeterioration and Biodegradation, 2016, 106:16–23. Ver publicación

Mahdy, A.; Ballesteros, M.; González-Fernández, C. Enzymatic pretreatment of Chlorella vulgaris for biogas production: Influence of urban wastewater as a sole nutrient source on macromolecular profile and biocatalyst efficiency. Bioresource Technology, 2016, 199:319–325. Ver publicación

Mahdy, A.; Méndez, L.; Tomás-Pejó, E.; Morales, M.M.; Ballesteros, M.; González-Fernández, C. Influence of enzymatic hydrolysis on the biochemical methane potential of Chlorella vulgaris and Scenedesmus sp. Journal of Chemical Technology and Biotechnology, 2016, 91(5):1299–1305. Ver publicación

Méndez, L.; Sialve, B.; Tomás-Pejó, E.; Ballesteros, M.; Steyer, J.P.; González-Fernández, C. Comparison of Chlorella vulgaris and cyanobacterial biomass: cultivation in urban wastewater and methane production. Bioprocess and Biosystems Engineering, 2016, 39(5):703–712. Ver publicación

Molinuevo-Salces, B.; Mahdy, A.; Ballesteros, M.; González-Fernández, C. From piggery wastewater nutrients to biogas: Microalgae biomass revalorization through anaerobic digestion. Renewable Energy, 2016, 96:1103–1110. Ver publicación

Moreno, A.D.; Ibarra, D.; Alvira, P.; Tomás-Pejó, E.; Ballesteros, M. Exploring laccase and mediators behavior during saccharification and fermentation of steam-exploded wheat straw for bioethanol production. Journal of Chemical Technology and Biotechnology, 2016, 91(6):1816-1825. Ver publicación

Moreno, A.D.; Ibarra, D.; Mialon, A.; Ballesteros, M. A bacterial laccase for enhancing saccharification and ethanol fermentation of steam-pretreated biomass. Fermentation, 2016, 2:11. Ver publicación

Oliva-Taravilla, A.; Tomás-Pejó, E.; Demuez, M.; González-Fernández, C.; Ballesteros, M. Effect of laccase dosage on enzymatic hydrolysis and fermentation of steam-exploded wheat straw. Cellulose Chemistry and Technology, 2016, 50(3-4):391–395. Ver publicación

Oliva-Taravilla, A.; Tomás-Pejó, E.; Demuez, M.; González-Fernández, C.; Ballesteros, M. Optimization of the laccase detoxification step in hybrid hydrolysis and fermentation processes from wheat straw by K. marxianus CECT 10875. Bioethanol, 2016, 2:126–133. Ver publicación

Oliva-Taravilla, A.; Tomás-Pejó, E.; Demuez, M.; González-Fernández, C.; Ballesteros, M. Phenols and lignin: key players in reducing enzymatic hydrolysis yields of steam-pretreated biomass in presence of laccase. Journal of Biotechnology, 2016, 218:94–101. Ver publicación

Demuez, M.; González-Fernández, C.; Ballesteros, M. Algicidal microorganisms and secreted algicides: New tools to induce microalgal cell disruption. Biotechnology Advances, 2015, 33(8):1615–1625. Ver publicación

Demuez, M.; Mahdy, A.; Tomás-Pejó, E.; González-Fernández, C.; Ballesteros, M. Enzymatic cell disruption of microalgae biomass in biorefinery processes. Biotechnology and Bioengineering, 2015, 112(10):1955–1966. Ver publicación

López-Linares, J.C.; Ballesteros Perdices, I.; Tourán, J.; Cara, C.; Castro, E.; Ballesteros, M.; Romero, I. Optimization of uncatalyzed steam explosion pretreatment of rapeseed straw for biofuel production. Bioresource Technology, 2015, 190:97–105. Ver publicación

Mahdy, A.; Méndez, L.; Ballesteros, M.; González-Fernández, C. Algaculture integration in conventional wastewater treatment plants: Anaerobic digestion comparison of primary and secondary sludge with microalgae biomass. Bioresource Technology, 2015, 184:236–244. Ver publicación

Mahdy, A.; Méndez, L.; Ballesteros, M.; González-Fernández, C. Protease pretreated Chlorella vulgaris biomass bioconversion to methane via semi-continuous anaerobic digestion. Journal of Chemical Technology and Biotechnology, 2015, 158:35–41. Ver publicación

Martínez-Patiño, J.C.; Romero-García, J.M.; Ruiz, E.; Oliva, J.M.; Álvarez, C.; Romero, I.; Negro, M.J.; Castro, E. High solids loading pretreatment of olive tree pruning with dilute phosphoric acid for bioethanol production by Escherichia coli. Energy and Fuels, 2015, 29:1735–1742. Ver publicación

Méndez, L.; Mahdy, A.; Ballesteros, M.; González-Fernández, C. Biomethane production using fresh and thermally pretreated Chlorella vulgaris biomass: A comparison of batch and semi-continuous feeding mode. Ecological Engineering, 2015, 84, 273–277. Ver publicacion

Méndez, L.; Mahdy, A.; Ballesteros, M.; González-Fernández, C. Chlorella vulgaris vs cyanobacterial biomasses: Comparison in terms of biomass productivity and biogas yield. Energy Conversion and Management, 2015, 92:137-142. Ver publicación

Moreno, A.D.; Ibarra, D.; Alvira, P.; Tomás-Pejó, E.; Ballesteros, M. A review of biological delignification and detoxification methods for lignocellulosic bioethanol production. Critical Reviews in Biotechnology, 2015, 35(3):342–354. Ver publicación

Negro, M.J.; Duque, A.; Manzanares, P.; Sáez, F.; Oliva, J.M.; Ballesteros, I.; Ballesteros, M. Alkaline twin-screw extrusion fractionation of olive-tree pruning biomass. Industrial Crops and Products, 2015, 74:336–341. Ver publicación

Oliva-Taravilla, A.; Moreno, A.D.; Ibarra, D.; Tomás-Pejó, E.; González-Fernández; Ballesteros, M. Unraveling the effects of laccase treatment on enzymatic hydrolysis of steam-exploded wheat straw. Bioresource Technology, 2015, 175:209–215. Ver publicación

Oliva-Taravilla, A.; Tomás-Pejó, E.; Demuez, M.; González-Fernández, C.; Ballesteros, M. Inhibition of cellulose enzymatic hydrolysis by laccase-derived compounds from phenols. Biotechnology Progress, 2015, 31(3):700–706. Ver publicación

San Martín-Davison, J.; Ballesteros, M.; Manzanares, P.; Petit-Breuilh, X.; Vergara, A. Effects of temperature on steam explosion pretreatment of poplar hybrids with different lignin contents in bioethanol production. International Journal of Green Energy, 2015, 12(8):832–842. Ver publicación

Santos, J.I.; Fillat, U.; Martín-Sampedro, R.; Ballesteros, I.; Manzanares, P.; Ballesteros, M.; Eugenio, M.E.; Ibarra, D. Lignin-enriched fermentation residues from bioethanol production of fast-growing poplar and forage sorghum. BioResources, 2015, 10(3):5215–5232. Ver publicación

Santos, J.I.; Martín-Sampedro, R.; Fillat, U.; Negro, M.J.; Oliva, J.M.; Ballesteros, M.; Eugenio, M.E.; Ibarra, D. Evaluating lignin-rich residues from biochemical ethanol production of wheat straw and olive tree pruning by FTIR and 2D-NMR. International Journal of Polymer Science, 2015, 2015:314891. Ver publicación

Moreno, A.D.; Ballesteros, M.; Negro, M.J. Biorefineries for the valorization of food processing waste. The Interaction of Food Industry and the Environment, 2020, pp. 155-190. Galanakis, C. (ed). Academic Press. ISBN: 978-0-12-816449-5.

Moreno, A.D.; Susmozas, A.; Oliva, J.M.; Negro, M.J. Overview of bio-based industries. Biobased Products and Industries, 2020, pp. 1-40. Galanakis, C.M. (ed). Elsevier. ISBN: 978-0-12-818493-6.

Ballesteros, M.; Manzanares, P. Liquid biofuels. The role of bioenergy in the emerging bioeconomy: Resources, technologies, sustainability and policy, 2019, pp. 113-144. Lago, C.; Caldés, N.; Lechón, Y. (eds). Academic Press, London, UK. ISBN: 978-0-12-813056-8.

Moreno, A.D.; Tomás-Pejó, E.; Ballesteros, M.; Negro, M.J. Pretreatment technologies for lignocellulosic biomass deconstruction within a biorefinery perspective. Biofuels: Alternative Feedstocks and Conversion Processes (2nd Edition), 2019, pp. 379-399. Pandey, A.; Larroche, C.; Gnansounou, E.; Khanal, S.K.; Dussap, C.G.; Ricke, S. (eds). Academic Press, London, UK. ISBN: 978-0-12-816856-1.

Marques, S.; Moreno, A. D.; Ballesteros, M.; Gírio, F. Starch biomass for biofuels, biomaterials, and chemicals. Biomass and Green Chemistry: Building a Renewable Pathway, 2018, pp. 69-94. Vaz Jr, S. (ed). Springer International Publishing, Switzerland. ISBN: 978-3-319-66736-2

González-Fernández, C.; Méndez, L.; Ballesteros, M.; Tomás-Pejó, E. Hydrothermal processing of microalgae. Hydrothermal Processing in Biorefineries - Production of Bioethanol and High Added-Value Compounds of Second and Third Generation Biomass, 2017, pp. 483-500. Ruiz, H.A; Trajano, H.; Mette, H. (eds). Springer Nature, Switzerland. ISBN: 978-3-319-56456-2.

Moreno, A.D.; Alvira, P.; Ibarra, D.; Tomás-Pejó, E. Production of ethanol from lignocellulosic biomass. Biofuels and Biorefineries: Production of Platform Chemicals from Sustainable Resources, 2017, pp. 375-410. Fang, Z.; Smith, R.L.; Qi, X. (eds). Springer Nature Singapore, Singapore. ISBN: 978-981-10-4171-6.

Moreno, A.D.; Olsson, L. Pretreatment of Lignocellulosic Feedstocks. Extremophilic Enzymatic Processing of Lignocellulosic Feedstocks to Bioenergy, 2017, pp. 31-52. Sani, R.K.; Krishnaraj, R.N. (eds). Springer Nature, Switzerland. ISBN: 978-3-319-54683-4.

Negro, M.J.; Manzanares, P.; Ruiz, E.; Castro, E.; Ballesteros, M. The biorefinery concept for the industrial valorization of residues from olive oil industry. Olive Mill Waste - Recent Advances for Sustainable Management, 2017, pp. 57-78. Galanakis, C.M. (ed). Academic Press, United Kingdom. ISBN: 978-0-12-805314-0.

Tomás-Pejó, E.; Moreno, A.D. Alcohol fuels: The biochemical route. Biofuels Production and Processing Technology, 2017, pp. 341-360. Riazi, M.R.; Chiaramonti, D. (eds). CRC Press, USA. ISBN: 9781498778930.

Duque, A.; Manzanares, P.; Ballesteros, I.; Ballesteros, M. Steam explosion as lignocellulosic biomass pretreatment. Biomass Fractionation Technologies for a Lignocellulosic Feedstock Based Biorefinery, 2016. Mussato, S.I. (ed). Elsevier, Netherlands. ISBN: 978-0-12-802323-5.

Ballesteros, M. Procesos bioquímicos: fermentación de biomasa azucarada y amilácea (bio-alcohol). Tecnologías para el uso y transformación de biomasa energética, 2015. San Miguel, G.; Martín, G. (eds). Mundi Prensa, España. ISBN: 978-84-8476-674-2.

WO2013182827. 12/12/2013. Vilarem, G.; Rigal, L.; Vandenbossche, V.; Brault, J.; Hernández-Luna, M.; Hernández-Meléndez, O.; Vivaldo-Lima E.; Barzana-García, E.; Ballesteros, M.; Duque, A.; Manzanares, P.; Siika-aho M.S.; Uusitalo, J.M.; Mata-Segreda, J.; Guillouet, S.; Lombard, E.; Cameleyre, X. Method for enzymatic treatment of a solid lignocellulosic material.

EP2112226. 15/02/2007. Signes, V.; Ballesteros, M.; Ballesteros, I.; Manzanares, P.; Martinez, J.M., Negro, M.J.; Oliva, J.M.; Coll, C.; Castañeda, R. Method for recovering energy from the organic fraction of solid urban waste and associated facility.

PCT/ES2003/000085. 17/07/2002. Ballesteros, M.; Negro, M.J.; Manzanares, P.; Ballesteros, I.; Oliva, J.M. Procedimiento para la extracción de compuestos fenólicos a partir de un material vegetal residual mediante un tratamiento hidrotérmico.

EP1130085. 24/02/2000. Ballesteros, I.; Ballesteros, M.; Oliva, J.M.; Carrasco, J. Procedimiento de producción de etanol a partir de biomasa lignocelulósica utilizando una nueva levadura termotolerante.